DNA exposed to UV light accumulates a number of DNA photoproducts, predominantly (6-4) photoproducts and cyclobutane pyrimidine dimers. Isolated DNA may be immobilized in 96 well microplates for analysis by ELISA. Intact cells or tissues may also be investigated using fluorescent immunohistochemical methods. The antibody is ideal for studies involving UV damage to skin or UV damage DNA repair mechanisms.
IMMUNOGEN:
The antibody was raised against UV irradiated calf thymus ssDNA conjugated to methylated BSA (mBSA) and UV irradiated poly dT-mBSA complex.
PHYSICAL STAT E :
The antibody is provided at 1 mg/ml as purified ascites containing 0.01% sodium azide.
I G C L A S S :
IgG1/κ
SPECIFICITY:
Specificity has been demonstrated for (6-4)-dithymidine, whereas the (6-4)-dicytidines or cyclo-butadipyrimidines are not recognized. A low level of cross reactivity was observed with repeating (6-4)-thymine/cytidines within an oligonucleotide sequence. The UVssDNA clone C3B6 antibody recognizes (6-4)-dipyrimidines in single stranded DNA at least 4 nucleotides long.
STORAGE:
Store at 4°C. For long term storage, freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.
APPLICAT I O N S :
ELISA and indirect in situ immunofluorescence to facilitate the study of the biological significance of DNA lesions. Related products include UVDE (catalog # 4100-100-EB), DermaTACS™ (catalog # 4829-30-K), EpiDerm™ Control Slides (catalog # 4800-30-02).
REFERENCES:
1. Liang X, Pickering MT, Cho N-H, Chang H, Volkert MR, Kowalik TF, and Jung JU. 2006. Deregulation of DNA Damage Signal Transduction by Herpesvirus Latency-Associated M2. J. Virol. 80:5862-74.
2. Strickland PT, Creasey JS. 1988. Immunoassay of dithymidine cyclobutane dimers in nanogram quantities of DNA. IARC Sci Publ. 89:341-4.
3. Strickland PT, Nikaido O, Matsunaga T, Boyle JM. 1992. Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts. Photochem Photobiol. 55:723-7.
4. Bruze M, Emmett EA, Creasey J, Strickland PT. 1989. Cyclobutadithymidine induction by solar-simulating UV radiation in human skin: II. Individual responses. J Invest Dermatol. 93:341-4.
5. Lesko SA, Li W, Zheng G, Callahan D, Kaplan DS, Midden WR, Strickland PT. 1989. Quantitative immunofluorescence assay for cyclobutyldithymidine dimers in individual mammalian cells. Carcinogenesis 10:641-6.
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